SPECIFIC SCAR PRIMERS FOR THE “FLAG SHOOT” AND “ASCOSPORE” BIOTYPES OF THE GRAPE POWDERY MILDEW FUNGUS ERYSIPHE NECATOR

H. Hajjeh, M. Miazzi, M.A. De Guido, F. Faretra
doi: 10.4454/jpp.v87i1.899
Abstract:
Eight RAPD amplicons selected previously as specific for isolates belonging to the “flag shoot” or the “ascospore” biotypes of Erysiphe necator were purified, cloned and sequenced. Thirty-seven SCAR (Sequence Characterised Amplified Region) primers were designed on the obtained sequences and tested for their specificity. Two pairs (UnE-UnF and F6-F6a) were specific for E. necator and effectively distinguished the two biotypes. Both primer pairs were tested on 374 E. necator isolates (83 “flag shoot” and 291 “ascospore”), already characterized by RAPD analysis, confirming their specificity and corroborating previous results. The primers are now being used to assess the biological significance and epidemiological role of the two biotypes in E. necator populations.
Vol. 87, N° 1 (2005)
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