Analysis of phenotypic and molecular diversity of phaeomoniella chlamydospora isolates in Spain

M.L. Tello, D. Gramaje, A. Gómez, P. Abad-Campos, J. Armengol
doi: 10.4454/jpp.v92i1.30
Phenotypic and molecular diversity of 57 Phaeomoniella chlamydospora isolates obtained in 2001-2005 from diverse Spanish viticultural areas and scion/rootstock combinations was investigated. Reference isolates of this species were included in the analysis. For phenotypic characterization, mycelial growth rates at 5, 10, 15, 20, 25, 30 and 35ºC, sporulation on potato dextrose agar (PDA) and production of the Phoma-like synanamorph on carnation leaf agar (CLA) were studied. Molecular characterization was performed using five Inter-Simple Sequence Repeat (ISSR) and 24 random amplified polymorphic DNA (RAPD) markers. Both phenotypic and molecular data showed strong homogeneity among all Spanish P. chlamydospora isolates, regardless of their geographical origin, year of isolation and scion/rootstock combination.