Occurrence of bacterial soft rot of pleurotus ostreatus caused by burkholderia gladioli pv agaricicola in Korea

C.J. Lee, H.S. Yun, C.S. Jhune, J.C. Cheong, Y.B. Yoo
doi: 10.4454/jpp.v92i1.36
An unknown bacterium was isolated from cultivated oyster mushrooms grown in Korea, which caused a soft rot that developed more rapidly than that elicited by the brown blotch bacterium, Pseudomonas tolaasii at temperatures above 25ºC. From these lesions a bacterial strain (designated OM1) was isolated which, following inoculation of mushroom caps, yielded characteristic sunken water-soaked brown spots, which developed into a severe soft rot. In the early stages, the lesions were indistinguishable from those of bacterial brown blotch, well known to mushroom growers. Results of Gram stain and biochemical tests identified the new bacterial isolate as Burkholderia gladioli pv. agaricicola. This was confirmed by pathogenicity to oyster mushroom, physiological and biochemical properties, analysis of the 16S rRNA gene sequences and the fatty acids profile. This is the first report of the isolation of B. gladioli pv. agaricicola from cultivated oyster mushroom in Korea.