PROPERTIES AND TAXONOMIC POSITION OF HOARY CRESS STRAIN OF CUCUMBER MOSAIC VIRUS
R. Rasoulpour, K. Izadpanah
A strain of Cucumber mosaic virus naturally infecting hoary cress (Lepidium draba) in Shiraz, Iran (designated CMV-Ld), induces vein clearing and mosaic on the leaves of naturally infected plants. Datura metel, D. stramonium, Lens culinaris, Lycopersicon esculentum, Nicotiana rustica and N. tabacum “Turkish” showed line pattern after mechanical inoculation with the virus. These symptoms were different from those induced by a cucumber strain of CMV (CMV-Cu). CMV-Ld was serologically similar to the cucumber strain in gel immunodiffusion tests using homologous and heterologous antisera. SDS-polyacrylamide gel electrophoresis of purified virus preparation yielded a protein band with molecular weight of 25 kDa. Analysis of the nucleotide sequence of the coat protein (CP) gene showed that CMV-Ld, CMV-Cu and other CMV strains could be assigned to subgroups on the basis of EcoRI restriction site. The full length sequences of the CP gene of both strains showed that CMV-Ld and CMV-Cu belong to subgroups II and IA, respectively.