Resistance gene cloning from a wild crop relative by sequence capture and association genetics
Nature Biotechnology
2019 - Vol. 37 - pag. 139–143 Authors: Sanu Arora, Burkhard Steuernagel, […], Brande B. H. Wulff
Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.
