YELLOWING DISEASE OF MELON IN SARDINIA ITALY CAUSED BY BEET PSEUDOYELLOWS VIRUS
L. Tomassoli, V. Lumia, G.F. Siddu, M. Barba
In 2001, a disease of melon characterized by yellowing of the leaves was observed in Sardinia (Italy) where it caused severe damage. Samples, collected from several melon crops, were positive in single step RT-PCR assays for Beet pseudoyellows virus (BPYV) when specific primers were used. An amplicon of the same size as that from BPYV controls (450 bp) was obtained from 14 out of 20 samples tested and had 98.4% nucleotide sequence identity with an authentic BPYV sequence. This is the first experimental evidence of a yellowing disease caused by BPYV in Italian melon crops.