DIFFERENTIATION OF DRECHSLERA GRAMINEA ISOLATES BY CULTURAL CHARACTERISTICS AND SDSPAGE
M.I.E. Arabi, N. MirAli, M. Jawhar, B. Alsafadi
Isolates of Drechslera graminea, the causal agent of the barley leaf stripe disease, were obtained from phytopathological samples collected in 1998 from different regions of Syria and analyzed for differences in cultural characteristics and protein profiles as determined by a 10% sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Differences in mycelia growth and colour were noticed among isolates collected from different regions. On the other hand, protein polymorphisms (SDS-PAGE) were detected, and highly significant differences (P < 0.001) for band numbers were obtained among isolates. The protein profiles were highly repeatable, suggesting that the biochemical SDS-PAGE protein markers are suitable to detect variability among D. graminea isolates.