DETECTION OF CITRUS PSOROSIS VIRUS BY DIRECT TISSUE BLOT IMMUNOASSAY
A.M. D’Onghia, K. Djelouah, D. Frasheri, O. Potere
Detection of Citrus psorosis virus (CPsV) by direct tissue blot immunoassay (DTBIA) was attempted using different tissues from CPsV-infected sources. Freshly cut surfaces of different plant organs were gently pressed on nitrocellulose membranes, exposed to a CPsV monoclonal antibody (MAb Ps29) conjugated with alkaline phosphatase, and stained with BCIP-NBT Sigma fast. For comparison, some explants were tested also by DAS-ELISA using the same MAb and a polyclonal antiserum for plate coating. Purple staining (positive reaction) was readily observed when flower explants were used, especially the ovary. DTBIA from ovaries correlated with DAS-ELISA, proving reliable and sensitive for the rapid detection of CPsV infections.