EFFECT OF CUCUMBER MOSAIC VIRUS MONOCLONAL ANTIBODIES ON VIRUS INFECTIVITY AND TRANSMISSION BY MYZUS PERSICAE
A. Gera, H.T. Hsu, J. Cohen, A. Watad, E. Beckelman, Y.H. Hsu
Seven affinity-purified monoclonal antibodies (mAbs) produced against Cucumber mosaic virus (CMV) subgroup I and II, were screened for their ability to neutralize virus infectivity in vitro. Infectivity was inhibited almost completely when virus preparations were mixed in vitro with the homologous mAb prior to inoculation. The most efficient mAbs were 4H10B12 and 6D11D12 for subgroups I and II, respectively. Aggregation of CMV virions following incubation with Abs was demonstrated using electron microscopy. The ability of the mAbs to block virus transmission by Myzus persicae was also tested; both, pre- and post-acquisition treatments of aphids with mAbs significantly decreased virus transmission. Treatment with mAb 6D11D12 before or after feeding on purified virus preparations prevented CMV transmission almost completely.