IDENTIFICATION AND PARTIAL CHARACTERIZATION OF TOMATO SPOTTED WILT VIRUS ON LETTUCE IN JORDAN
N.M. Salem, A. Mansour, H. Badwan
Tomato spotted wilt virus (TSWV) was isolated from naturally infected lettuce grown in Jordan. This virus isolate (TSWV-J) was identified based on transmission studies, host range reaction, and serological tests. RTPCR was used for the detection of TSWV in infected plant tissues. Total RNA isolated from infected lettuce plants was subjected to RT-PCR using primers specific to the nucleocapsid (N) gene of TSWV and the amplified 620 bp product was cloned and sequenced. Nucleotide sequence and phylogenetic analysis of the RTPCR amplicon confirmed the Jordanian virus as an isolate of TSWV. Furthermore, the genetic diversity of TSWV in Jordan was investigated. The TSWV isolates shared high nucleotide sequence identity (93-99%) with those from other countries. To our knowledge, this is the first report of TSWV infection on lettuce in Jordan.