DEVELOPMENT OF A OLIGONUCLEOTIDEBASED MICROARRAY FOR DETECTION OF MULTIPLE ARTICHOKE VIRUSES
A. Tiberini, M. Barba
Globe artichoke (Cynara scolymus) is a vegetable crop affected by many viruses causing significant economic losses. Virus detection and identification is important to farmers, plant pathologists and others involved in plant protection activities of quarantine and certification programs. To this aim, the potentiality of an oligonucleotide diagnostic array based on the Combimatrix platform for the detection and identification of 14 artichoke-infecting viruses was examined. More than 190 oligonucleotide virus probes (40-mer) were selected and used in the microarray diagnostic technique developed in this investigation. Most of the probes were highly specific for detection, identification and discrimination of target viruses. Further, for the first time the Combimatrix platform was applied directly to evaluate the sanitary status of artichoke samples collected during field surveys carried out in 6 different Italian regions. The repeatability, specificity and sensitivity of the oligonucleotide diagnostic array developed in this investigation demonstrated its applicability for routine diagnostic use in artichoke germplasm as it detected simultaneously 14 viruses in one single hybridization event.