HIGH DIVERSITY OF THE COAT PROTEIN GENE SEQUENCE OF NARCISSUS LATENT VIRUS ISOLATES
H. Berniak, B. Komorowska, D. Sochacki, B. Hasiów-Jaroszewska
Narcissus latent virus (NLV), a member of the genus Macluravirus, is common in many commercially important narcissus cultivars worldwide. In this study, the nucleotide sequence of the complete coat protein (CP) gene of 14 NLV isolates recovered from naturally infected narcissus plants was determined and compared with three other NLV isolates for which sequence information is available. Multiple alignments showed sequence identities ranging from 77.2 to 79.1% and 77.9 to 81.2% at the nucleotide and deduced amino acid levels, respectively. Phylogenetic analysis based on CP gene sequences confirmed a high variability of NLV isolates and showed that they form two distinct groups. Further analyses suggested a negative selection as the major force generating genetic variability of NLV capsid protein, however, codon 41 was under positive selection. This study provides new insights into the genetic variability of NLV CP gene.