CHINA ROSE HIBISCUS ROSASINENSIS: A NEW NATURAL HOST OF COTTON LEAF CURL BUREWALA VIRUS IN PAKISTAN
K.P. Akhtar, R. Ullah, M. Saeed, N. Sarwar, S. Mansoor
China rose (Hibiscus rosa-sinensis) is a perennial ornamental plant grown throughout the tropics and subtropics. China rose plants with severe vein thickening/ greening, leaf curling, and enations on the lower leaf surface were found near cotton fields at the Nuclear Institute of Agriculture Biology (NIAB) in Faisalabad. The symptoms of disease exhibited on China rose plants were very much similar to Cotton leaf curl disease exhibited by cotton plants. DNA was extracted from ten naturally infected symptomatic China rose plants and subjected to PCR using begomovirus and betasatellite specific primers. The expected products of 2.8Kb and 1.4Kb for begomovirus and betasatellites were amplified, respectively. The begomovirus and its cognate betasatellite isolated from China rose were sequenced and submitted to the database with accession numbers HG003876 and HG003877, respectively. Owing to a 99% nucleotide sequence identity, this virus was designated as an isolate of Cotton leaf curl Burewala virus (CLCuBuV). Similarly the sequenced betasatellite showing 96% nucleotide sequence identity was designated as an isolate of Cotton leaf curl Multan betasatellite (CLCuMuB). The symptomatic induction of Cotton leaf curl disease (CLCuD) by indexing in LRA-5166, a CLCuBuV susceptible genotype of cotton that resists Cotton leaf curl Multan virus (CLCuMuV), further confirmed the presence of CLCuBuV in China rose. To our knowledge, this is the first report of CLCuBuV and its cognate betasatellite in infected China rose.