Archivio Journal of Plant Pathology

Abstract:

During 2006 and 2007, 110 Streptomyces strains were isolated from potato tubers with various symptoms including erumpent, deep-pitted and superficial scab lesions from different regions of Fars and Hamedan provinces of Iran. The isolates were identified as Streptomyces scabies, S. acidiscabies, S. turgidiscabies, S. aureofaciens, S. reticuliscabies, S. griseus, S. europaeiscabies and S. stelliscabies using physiological, biochemical and morphological tests and were classified into six groups using the NTSYSpc version 2.02e software. The isolates were divided into nine clusters based on the numerical analysis of rep-PCR using Box primers. Clustering based on phenotypic features confirmed. In some cases, the clustering based on rep-PCR with Box primers. Rep-PCR DNA fingerprints of Streptomyces strains with BoxA1R primers were relatively unique, consistent, and reproducible. Our results show that Box-PCR is a very useful tool for a quick determination of strain identity.