IDENTIFICATION AND CHARACTERIZATION OF AN OLIVE LATENT VIRUS 1 ISOLATE FROM A NEW HOST: SOLANUM LYCOPERSICUM
N. Borodynko, B. Hasiów-Jaroszewska, H. Pospieszny
Isometric virus particles with a diameter of approximately 30 nm were observed in the Wielkopolska region of Poland in greenhouse-grown tomato plants with necrotic foliar symptoms. The viral genome consisted of a single-stranded RNA molecule. RT-PCR with random hexamer primers and sequencing of DNA products revealed 91% identity to Olive latent virus 1 (OLV-1). Further RT-PCR with virus-specific primers confirmed the presence of OLV-1 in symptomatic plants. The host range and the sequence of the coat protein gene of the new OLV-1 isolate, designated CM1, was determined. Differences were observed in biological and genetic traits between the Polish isolate CM1 and other OLV-1 isolates. To our knowledge, this is the first report of OLV-1 in tomato plants.