DETECTION OF BEET SOILBORNE VIRUS AND BEET VIRUS Q IN SUGARBEET IN GREECE
O.I. Pavli, M. Prins, G.N. Skaracis
Sugar beet plants with typical rhizomania symptoms were collected from the five major cultivation zones of Greece. The presence of Beet necrotic yellow vein virus (BNYVV), the primary causal agent of the disease, was ascertained by DAS-ELISA in 38 out of 40 fields surveyed and the positive samples were subsequently examined for the presence of other soil-borne viruses which are frequently associated with rhizomania, using a multiplex RT-PCR assay targeting BNYVV, Beet soilborne virus (BSBV) and Beet virus Q (BVQ). The occurrence of BSBV and BVQ was confirmed in 9 and 23 rhizomania- infected fields, respectively. In contrast to surveys conducted in other countries, the presence of BVQ prevailed throughout Greece in dual infections with BNYVV, whereas BSBV was restricted to rhizomaniainfected fields from only two sugarbeet cultivation areas. Nine of the samples tested were infected with all three viruses. BSBV was always found in triple infections. To our knowledge, this is the first report of BSBV and BVQ in Greece. The future assessment of the impact of each of these viruses on sugarbeet could prove significant for breeding objectives in terms of achieving a more durable resistance to the rhizomania syndrome.