HIPPEASTRUM MOSAIC VIRUS DIAGNOSED IN HIPPEASTRUM AND EUCHARIS IN BRAZIL

M.A.V. Alexandre, L.M.L. Duarte, E.B. Rivas, A. Cilli, R. Harakava, S.R. Galleti, E.W. Kitajima
doi: 10.4454/jpp.v93i3.3646
Abstract:
A potyvirus associated with foliar mosaic symptoms displayed by Hippeastrum (Hip-Br) and two Eucharis samples (Euch-B and Euch-C) from three different cities of Brazil was detected and identified by transmission tests, electron microscopy, reverse-transcription, RT-PCR and sequencing. Transmission electron microscopy revealed the presence of elongated, flexuous viral particles in the extracts of symptomatic leaves, while in thin sections of symptomatic leaves, cytoplasmic aggregates of type II lamellar inclusions (Edwardson’s classification) were observed. The experimental host range was restricted. RT-PCR using universal potyvirus primers amplified DNA products of 1,499 bp corresponding to the partial nuclear inclusion body (NIb) gene, full-length coat protein (CP) gene and 3’end untranslated region (UTR). Pairwise analyses from CP and NIb nucleotide sequences of Hip-Br, Euch-B and Euch-C viruses and other potyviruses revealed high identities with previously reported Alaska (GQ857550) and Rilona (EF203685) Hippeastrum mosaic virus (HiMV) isolates, Amazon lily mosaic virus (ALiMV, AY590143), and Amaryllis potyvirus (AmaPV, AY566239). The phylogeny calculated by a maximum likelihood method showed that Hip-Br, Euch-B and Euch-C formed a monophyletic group supported by 100% bootstrap with HiMV. This is the first report of HiMV in Hippeastrum sp. and Eucharis sp. from Brazil. Our data support the taxonomic position of HiMV and other potyviruses isolated from amaryllis and Amazon lily in a separated cluster of Amazon lily mosaic virus (AB158523).
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