EXPLORING GENE INDICATORS TO PREDICT SUSCEPTIBILITY TO SCLEROTINIA SCLEROTIORUM IN BRASSICA PLANTS
Y.H. Li, Y.J. Ding, C.G. Shao, J.Q. Mei, W. Qian
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease in Brassica spp. Here we proposed a method to indicate the susceptibility of Brassica spp. or Brassica genus against S. sclerotiorum by detecting expression of a gene from host with qRT-PCR technique. After filtering a set of transcriptome data from B. oleracea leaf infected by S. sclerotiorum, twelve genes that exhibited successive increase or decrease expression after inoculation were chosen to screen the dynamic expression changes in S. sclerotiorum-inoculated B. napus leaf. Among the genes tested, Bol024541 showed a continuous increase from the outer region to the inoculation site from 12 to 36 hours after infection (hai). No significant difference was found in qRT-PCR for the expression of Bol024541 in the samples added with various amount of cDNA of S. sclerotiorum, and significant linear correlation (r = 0.921, P < 0.01) was detected for the expression of Bol024541 at 12 hai with the lesion size at 72 hai among six Brassica accessions. Our data suggest that Bol024541 may be considered as an indicator to predict susceptibility to S. sclerotiorum in Brassica spp. via qRT-PCR.