FIRST REPORT OF TOMATO INFECTIOUS CHLOROSIS VIRUS IN GLOBE ARTICHOKE ECOTYPES IN APULIA SOUTHERN ITALY
R. Spanò, A. Corrado, A. Di Franco
Apulia (southern Italy) hosts a rich variety of globe artichoke ecotypes of high interest for breeding programs and industrial production. Most of the ecotypes are at risk of genetic erosion because of the high incidence of plant pathogens, which include infections from taxonomically different viruses. In the early autumn of 2014 and 2016 five samples were collected from each of the early-flowering ecotypes, Brindisino, Carciofo di Lucera, Francesina di Brindisi, Francesina di Trinitapoli, Locale di Mola, Nero di Ostuni, Tricasino, Troianella, Verde di Putignano, Violetto di Putignano and the late-flowering ecotypes Bianco di Taranto and Locale di Mola tardivo, and tested for viral infections by dot blot hybridization with Dig-labeled DNA probes and RT-PCR (Minutillo et al., 2012). In the five samples of the ecotypes Verde di Putignano, Violetto di Putignano, Nero di Ostuni and Troianella the hybridization signal revealed the presence of tomato infectious chlorosis virus (TICV) that in the ecotype Troianella was in mixed infection with artichoke Italian latent virus and turnip mosaic virus. TICV infection was confirmed by RT-PCR with TICV-specific primer pair for 5'- TCAGTGCGTACGTTAATGGG-3' and rev 5'-CACAGTATACAGCAGCGGCAG-3' (Minutillo et al. 2012). Amplicons from the ecotypes Verde di Putignano and Troianella were sequenced. Nucleotide sequence analyses revealed 99% identity with a TICV isolate from USA (accession No. FJ542306). Previous surveys detected TICV in globe artichoke in Liguria, Sardinia, Latium, Campania and Sicily but this is the first report of TICV in artichoke in Apulia and in the ecotypes Verde di Putignano, Violetto di Putignano, Nero di Ostuni and Troianella. Artichoke plants infected by TICV did not show symptoms and thus may act as perennial TICV reservoirs.