EXPERIMENTS ON TRANSMISSION OF PLUM POX VIRUS THROUGH PRUNUS SEEDS
S. Milusheva, P. Gercheva, V. Bozhkova, I. Kamenova
doi: 10.4454/jpp.v90i1sup.611
Abstract:
Vertical transmission of Plum pox virus (PPV) was investigated in seeds collected from three plum (Prunus domestica L.) cultivars infected with PPV-M, one apricot (Prunus armeniaca L.) cultivar infected with PPV-Rec and Prunus mahaleb L. The virus strains were identified before the beginning of the study. The presence of PPV in mature seeds and their parts, as well as in germinating seeds and seedlings was detected by ELISA. The highest percentage of infected whole seeds was registered in cv. Tuleu timpuriu (32%), followed by cvs Stanley (26%), Modesto (24%), and Valjevka (14%). The lowest infection rate was detected in P. mahaleb (11%). In the Prunus cultivars under study, PPV was detected in 19% to 35% of the seed coats and in 5% to 23% of the cotyledons. None of the embryonic axes was PPV-positive. In germinating seeds, PPV was detected in up to 34% of the seed coats and in up to 8.3% of the cotyledons. The virus was not identified in radicles and plumules. Up to now, all planted seedlings originating from seeds of plum cvs Tuleu tumpuriu, Stanley and Valjevka infected by PPV-M, and from seeds of apricot cv. Modesto infected with PPV-Rec, remained symptomless and reacted negatively with an antiserum to PPV.
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