A NOVEL INOCULATION METHOD FOR EVALUATION OF GREY LEAF SPOT RESISTANCE IN ITALIAN RYEGRASS

W. Takahashi, Y. Miura, T. Sasaki
doi: 10.4454/jpp.v91i1.638
Abstract:
We developed a novel inoculation method, the ‘filterpaper method’, for the assay of grey leaf spot (GLS) in Italian ryegrass (Lolium multiflorum Lam.). In this method, a conidial suspension of Pyricularia oryzae, the causal agent of GLS, is dropped onto a filter paper, which is placed, inoculated-side down, on the abaxial surface of a detached leaf on an agar plate. The filter-paper method was more repeatable than the spot inoculation method developed for rice, and the disease scores obtained were correlated (r = 0.6, P < 0.01) with those obtained under glasshouse conditions by the standard inoculation method. We optimized this novel method and demonstrated its usefulness for the selection of GLS resistance in Italian ryegrass.
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