MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV SYRINGAE STRAINS FROM DIFFERENT HOST PLANTS USING FLUORESCENT AMPLIFIED FRAGMENT LENGTH POLYMORPHISM
G. Cirvilleri, G. Scuderi, A. Bonaccorsi, M. Scortichini
The ability of fluorescent Amplified Fragment Length Polymorphism (fAFLP) technique for typing Pseudomonas syringae pv. syringae strains isolated from different host plants was investigated. Fragments up to 700 bp were separated on a CEQ 8000 automated DNA sequencer and electropherograms were generated with CEQ 8000 analysis software. Different patterns were generated from 57 P. s. pv. syringae strains examined and seven fAFLP clusters partially corresponding to the host of origin were identified. The analysis of the electropherograms allowed the identification of host-specific discriminative peaks. One peak was present in 93% of the analyzed strains. FAFLP analysis showed an high genetic heterogeneity in the P. s. pv. syringae strains examined, including strains isolated from the same host.