Journal of Plant Pathology Archive

Abstract:

During a survey conducted in summer 2010, severe virus symptoms including mosaic, mottling and leaf malformation were observed on Ibicella lutea in fields of the Varamin region in the Tehran province of Iran. Four symptomatic samples were collect- ed and leaf extracts were tested by DAS-ELISA for the presence of Arabis mosaic virus (ArMV), Cucumber mosaic virus (CMV), Potato leaf roll virus (PLRV), Potato virus Y (PVY), Squash leaf curl virus (SLCV), Tomato bushy stunt virus (TBSV), Tomato ringspot virus (ToRSV), Tomato spotted wilt virus (TSWV), Toma- to yellow leaf curl virus (TYLCV), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV), using com- mercial kits (Bioreba, Switzerland). None of the samples reacted with any of the antisera, except for that to CMV. The occurrence of CMV was confirmed by bioassays on herbaceous plants and by RT-PCR using total RNA isolated with TRI-Reagent (Sigma, USA) and CMV-specific primers designed on the coat protein gene (Blas et al., 1994). An amplicon of the expected size (540 bp) was obtained from symptomatic but not from apparently healthy samples. Comparisons of the nucleotide sequence of the coat protein gene of a CMV isolate from I. lutea designated CMV-ALF (accession No. JF343525) with other sequences avail- able in GenBank revealed 99% and 98% identity with CMV-Cu (EF620777) and CMV-CS (D28489), respectively. Furthermore, phylogenetic analysis showed that isolate ALF belongs to CMV group IA. This is the first report of CMV on I. lutea in Iran.