HETEROGENEITY IN PYRENOPHORA GRAMINEA AS REVEALED BY ITSRFLP
M.I.E. Arabi, M. Jawhar
doi: 10.4454/jpp.v89i3.770
Abstract:
Fifty-six isolates of Pyrenophora graminea, the causal agent of barley leaf stripe disease, were collected from different sites in Syria and analyzed using restriction digestion of PCR-amplified internal transcribed spacers (ITS) of ribosomal DNA. A total of 594 bands were scored of which 453 (76.3%) were polymorphic. The fingerprints generated from the six restriction digestions of the nrDNA ITS region showed high levels of intraspecific variation within the P. graminea population. A neighbour-joining diagram, based on Nei’s genetic distances, showed that isolates formed two phylogenetic groups, and did not fall into clusters or clades specific to the origin or colour of the isolate, which suggests a regional dispersal of P. graminea. The molecular parameter used in this study provides crucial information for studying genetic variation in P. graminea and provides important information for future selection of isolates to develop durable barley leaf stripe resistance.
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