MOLECULAR V ARIABILITY OF PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES FROM DIFFERENT PRUNUS HOSTS IN TUNISIA

N. Mahfoudhi, I. Selmi, A. Lehad, M. Elair, H. Ben Hamda, M.B. Allagui
doi: 10.4454/JPP.V97I1.031
Abstract:
Prunus necrotic ringspot virus (PNRSV) was detected by PCR in Tunisian stone fruit germplasm collections. Peach had the highest infection rate reaching 50%, followed by apricot (46.8%), almond (35.5%) and plum (9.5%). The genetic variability of the CP gene of 10 P N R S V isolates was evaluated by SSCP analysis of PCR products and analysis of their sequence. I n phylogenetic trees, Tunisian PNRSV isolates c l u s t e r e d in the three major groups, PV96, PV32 and PE5. However, an almond isolate (AlTun8) segregated as an out-group. This isolate is phylogenetically distant from other PNRSV isolates from different parts of the world and displays nucleic and amino acid identities of 83-86% and 65-74%, respectively, with PV96, PE5 and PV32. This is the first report on the molecular variability of PNRSV in different Prunus hosts in Tunisia.
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